113Cd-NMR investigation of a cadmium-substituted copper, zinc-containing superoxide dismutase from yeast

Pauli Kofod, Rogert Bauer, Eva Danielsen, Erik Larsen, Morten J. Bjerrum

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113Cd nuclear magnetic resonance spectroscopy has been used to investigate the metal binding sites of cadmium-substituted copper,zinc-containing superoxide dismutase from baker's yeast. NMR signals were obtained for 113Cd(II) at the Cu site as well as for 113Cd(II) at the Zn site. The two subunits in the dimeric enzyme were found to have identical coordination properties towards 113Cd(II) as only two 113Cd signals were found in 113Cd2113Cd2SOD. The difference between the chemical shifts of 113Cd(II) at the Zn site when no copper is coordinated at the Cu site, and when Cu(I) or Cd(II) is coordinated, were found to be very small indicating that 113Cd(II) must be bound to the same number and type of ligands in both cases. Furthermore, the spectra show that the rate of exchange of protein-bound 113Cd(II) and free 113Cd2+ is slow on the NMR time scale also at the Cu site. The present study suggests an explanation for the discrepancy in the literature regarding 113Cd-NMR investigations of bovine superoxide dismutase.
TidsskriftEuropean Journal of Biochemistry
Udgave nummer3
Sider (fra-til)607-611
Antal sider5
StatusUdgivet - 1 jun. 1991