Anti-CRISPR protein applications: natural brakes for CRISPR-Cas technologies

Nicole D Marino, Rafael Pinilla-Redondo, Bálint Csörgő, Joseph Bondy-Denomy

Publikation: Bidrag til tidsskriftReviewpeer review

Abstract

Clustered, regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) genes, a diverse family of prokaryotic adaptive immune systems, have emerged as a biotechnological tool and therapeutic. The discovery of protein inhibitors of CRISPR-Cas systems, called anti-CRISPR (Acr) proteins, enables the development of more controllable and precise CRISPR-Cas tools. Here we discuss applications of Acr proteins for post-translational control of CRISPR-Cas systems in prokaryotic and mammalian cells, organisms and ecosystems.

OriginalsprogEngelsk
TidsskriftNature Methods
Vol/bind17
Udgave nummer5
Sider (fra-til)471-479
Antal sider9
ISSN1548-7091
DOI
StatusUdgivet - maj 2020

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