TY - JOUR
T1 - Anti-CRISPR protein applications
T2 - natural brakes for CRISPR-Cas technologies
AU - Marino, Nicole D
AU - Pinilla-Redondo, Rafael
AU - Csörgő, Bálint
AU - Bondy-Denomy, Joseph
N1 - Publisher Copyright: © 2020, Springer Nature America, Inc.
PY - 2020/5/1
Y1 - 2020/5/1
N2 - Clustered, regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) genes, a diverse family of prokaryotic adaptive immune systems, have emerged as a biotechnological tool and therapeutic. The discovery of protein inhibitors of CRISPR-Cas systems, called anti-CRISPR (Acr) proteins, enables the development of more controllable and precise CRISPR-Cas tools. Here we discuss applications of Acr proteins for post-translational control of CRISPR-Cas systems in prokaryotic and mammalian cells, organisms and ecosystems.
AB - Clustered, regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) genes, a diverse family of prokaryotic adaptive immune systems, have emerged as a biotechnological tool and therapeutic. The discovery of protein inhibitors of CRISPR-Cas systems, called anti-CRISPR (Acr) proteins, enables the development of more controllable and precise CRISPR-Cas tools. Here we discuss applications of Acr proteins for post-translational control of CRISPR-Cas systems in prokaryotic and mammalian cells, organisms and ecosystems.
KW - Animals
KW - CRISPR-Associated Proteins/antagonists & inhibitors
KW - CRISPR-Cas Systems
KW - Enzyme Inhibitors/pharmacology
KW - Gene Editing
KW - Gene Expression Regulation
KW - Humans
KW - Protein Engineering
UR - http://www.scopus.com/inward/record.url?scp=85082704813&partnerID=8YFLogxK
U2 - 10.1038/s41592-020-0771-6
DO - 10.1038/s41592-020-0771-6
M3 - Review article
C2 - 32203383
SN - 1548-7091
VL - 17
SP - 471
EP - 479
JO - Nature Methods
JF - Nature Methods
IS - 5
ER -