Cardiac C-type natriuretic peptide gene expression and plasma concentrations in neonatal piglets

Lasse H Hansen, Julie Smith, Jens Peter Goetze

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    Abstract

    BACKGROUND:
    C-type natriuretic peptide (CNP) is a member of the natriuretic peptide family. Cardiac ANP and BNP expressions are firmly established, whereas CNP expression in the mammalian heart remains controversial. In the present report, we used a porcine model of the neonatal period with high expressions of cardiac ANP and BNP in order to elucidate the cardiac CNP expression profile.

    METHODS:
    Plasma and cardiac tissue were obtained from newborn piglets during the first 72h of life. The chamber-specific CNP mRNA contents were quantified by real-time PCR analysis. The proCNP concentrations in plasma and cardiac tissue extracts were quantified by a porcine-specific radioimmunoassay.

    RESULTS:
    Cardiac CNP mRNA contents (n=24) were low compared to sites of known expression, where porcine seminal vesicle CNP mRNA contents were 200-fold higher. In addition, plasma proCNP concentrations in the newborn piglets (n=44) were exceedingly low compared to proANP concentrations (5.3pmol/L (3.2-8.6) vs. 3438pmol/L (2790-5418), p<0.0001). The proCNP concentrations in atrial tissue extracts were barely detectable (≤0.06pmol/g) (n=2) compared to ventricular proANP (130pmol/g (101-159)) and atrial proANP (12,303pmol/g (10,623-15,412)).

    CONCLUSION:
    Our data show that the heart is not a major source of circulating proCNP in neonatal piglets.
    OriginalsprogEngelsk
    TidsskriftRegulatory peptides
    Vol/bind188
    Sider (fra-til)66-69
    ISSN0167-0115
    DOI
    StatusUdgivet - 10 jan. 2014

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