Chemically modified oligonucleotides with efficient RNase H response

Birte Vester; Anne Marie Boel; Sune Lobedanz; B Ravindra Babu; Michael Raunkjaer; Dorthe Lindegaard; Raunak; Patrick J Hrdlicka; Torben René Højland; Pawan K Sharma; Surender Kumar; Jesper Wengel; Poul Nielsen

doi:10.1016/j.bmcl.2008.03.004

Chemically modified oligonucleotides with efficient RNase H response

Birte Vester
, Anne Marie Boel
, Sune Lobedanz
, B Ravindra Babu
, Michael Raunkjaer
, Dorthe Lindegaard
, Raunak
, Patrick J Hrdlicka
, Torben René Højland
, Pawan K Sharma
, Surender Kumar
, Jesper Wengel
, Poul Nielsen

Syddansk Universitet

Publikation: Bidrag til tidsskrift › Tidsskriftsartikel › Forskning › peer review

Abstract

Ten different chemically modified nucleosides were incorporated into short DNA strands (chimeric oligonucleotides ON3-ON12 and ON15-ON24) and then tested for their capacity to mediate RNAse H cleavage of the complementary RNA strand. The modifications were placed at two central positions directly in the RNase H cleaving region. The RNA strand of duplexes with ON3, ON5 and ON12 were cleaved more efficiently than the RNA strand of the DNA:RNA control duplex. There seems to be no correlation between the thermal stability between the duplexes and RNase H cleavage.

Originalsprog	Engelsk
Tidsskrift	Bioorganic & Medicinal Chemistry Letters
ISSN	0960-894X
DOI	https://doi.org/10.1016/j.bmcl.2008.03.004
Status	Udgivet - 1 apr. 2008
Udgivet eksternt	Ja

Adgang til dokumentet

10.1016/j.bmcl.2008.03.004

Andre filer og links

https://portal.findresearcher.sdu.dk/en/publications/f04a5030-4e5d-11dd-b1a1-000ea68e967b

Citationsformater

@article{c3003dcec4534e9d90d4f7e4ea2fc522,

title = "Chemically modified oligonucleotides with efficient RNase H response",

abstract = "Ten different chemically modified nucleosides were incorporated into short DNA strands (chimeric oligonucleotides ON3-ON12 and ON15-ON24) and then tested for their capacity to mediate RNAse H cleavage of the complementary RNA strand. The modifications were placed at two central positions directly in the RNase H cleaving region. The RNA strand of duplexes with ON3, ON5 and ON12 were cleaved more efficiently than the RNA strand of the DNA:RNA control duplex. There seems to be no correlation between the thermal stability between the duplexes and RNase H cleavage.",

author = "Birte Vester and Boel, \{Anne Marie\} and Sune Lobedanz and Babu, \{B Ravindra\} and Michael Raunkjaer and Dorthe Lindegaard and Raunak and Hrdlicka, \{Patrick J\} and H{\o}jland, \{Torben Ren{\'e}\} and Sharma, \{Pawan K\} and Surender Kumar and Jesper Wengel and Poul Nielsen",

year = "2008",

month = apr,

day = "1",

doi = "10.1016/j.bmcl.2008.03.004",

language = "English",

journal = "Bioorganic \& Medicinal Chemistry Letters",

issn = "0960-894X",

publisher = "Elsevier Ltd",

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TY - JOUR

T1 - Chemically modified oligonucleotides with efficient RNase H response

AU - Vester, Birte

AU - Boel, Anne Marie

AU - Lobedanz, Sune

AU - Babu, B Ravindra

AU - Raunkjaer, Michael

AU - Lindegaard, Dorthe

AU - Raunak, null

AU - Hrdlicka, Patrick J

AU - Højland, Torben René

AU - Sharma, Pawan K

AU - Kumar, Surender

AU - Wengel, Jesper

AU - Nielsen, Poul

PY - 2008/4/1

Y1 - 2008/4/1

N2 - Ten different chemically modified nucleosides were incorporated into short DNA strands (chimeric oligonucleotides ON3-ON12 and ON15-ON24) and then tested for their capacity to mediate RNAse H cleavage of the complementary RNA strand. The modifications were placed at two central positions directly in the RNase H cleaving region. The RNA strand of duplexes with ON3, ON5 and ON12 were cleaved more efficiently than the RNA strand of the DNA:RNA control duplex. There seems to be no correlation between the thermal stability between the duplexes and RNase H cleavage.

AB - Ten different chemically modified nucleosides were incorporated into short DNA strands (chimeric oligonucleotides ON3-ON12 and ON15-ON24) and then tested for their capacity to mediate RNAse H cleavage of the complementary RNA strand. The modifications were placed at two central positions directly in the RNase H cleaving region. The RNA strand of duplexes with ON3, ON5 and ON12 were cleaved more efficiently than the RNA strand of the DNA:RNA control duplex. There seems to be no correlation between the thermal stability between the duplexes and RNase H cleavage.

UR - https://portal.findresearcher.sdu.dk/en/publications/f04a5030-4e5d-11dd-b1a1-000ea68e967b

U2 - 10.1016/j.bmcl.2008.03.004

DO - 10.1016/j.bmcl.2008.03.004

M3 - Journal article

C2 - 18356048

SN - 0960-894X

JO - Bioorganic & Medicinal Chemistry Letters

JF - Bioorganic & Medicinal Chemistry Letters

ER -