D-Allose catabolism of Escherichia coli: involvement of alsI and regulation of alsl regulation expression by allose and ribose

Tim S. Poulsen, Ying-Ying Chang, Bjarne Hove-Jensen

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    Abstract

    Genes involved in allose utilization of Escherichia coli K-12 are organized in at least two operons, alsRBACE and alsI, located next to each other on the chromosome but divergently transcribed. Mutants defective in alsI (allose 6-phosphate isomerase gene) and alsE (allulose 6-phosphate epimerase gene) were Als(-). Transcription of the two allose operons, measured as beta-galactosidase activity specified by alsI-lacZ(+) or alsE-lacZ(+) operon fusions, was induced by allose. Ribose also caused derepression of expression of the regulon under conditions in which ribose phosphate catabolism was impaired.
    OriginalsprogEngelsk
    TidsskriftJournal of Bacteriology
    Vol/bind181
    Udgave nummer22
    Sider (fra-til)7126-7130
    ISSN0021-9193
    StatusUdgivet - 1999

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