Genes involved in allose utilization of Escherichia coli K-12 are organized in at least two operons, alsRBACE and alsI, located next to each other on the chromosome but divergently transcribed. Mutants defective in alsI (allose 6-phosphate isomerase gene) and alsE (allulose 6-phosphate epimerase gene) were Als(-). Transcription of the two allose operons, measured as beta-galactosidase activity specified by alsI-lacZ(+) or alsE-lacZ(+) operon fusions, was induced by allose. Ribose also caused derepression of expression of the regulon under conditions in which ribose phosphate catabolism was impaired.
|Tidsskrift||Journal of Bacteriology|
|Status||Udgivet - 1999|
Poulsen, T. S., Chang, Y-Y., & Hove-Jensen, B. (1999). D-Allose catabolism of Escherichia coli: involvement of alsI and regulation of alsl regulation expression by allose and ribose. Journal of Bacteriology, 181(22), 7126-7130.