D-Allose catabolism of Escherichia coli: involvement of alsI and regulation of alsl regulation expression by allose and ribose

Tim S. Poulsen, Ying-Ying Chang, Bjarne Hove-Jensen

    Publikation: Bidrag til tidsskriftTidsskriftsartikelForskningpeer review


    Genes involved in allose utilization of Escherichia coli K-12 are organized in at least two operons, alsRBACE and alsI, located next to each other on the chromosome but divergently transcribed. Mutants defective in alsI (allose 6-phosphate isomerase gene) and alsE (allulose 6-phosphate epimerase gene) were Als(-). Transcription of the two allose operons, measured as beta-galactosidase activity specified by alsI-lacZ(+) or alsE-lacZ(+) operon fusions, was induced by allose. Ribose also caused derepression of expression of the regulon under conditions in which ribose phosphate catabolism was impaired.
    TidsskriftJournal of Bacteriology
    Udgave nummer22
    Sider (fra-til)7126-7130
    StatusUdgivet - 1999