Easy and efficient (111)Indium labeling of long-term stored DTPA conjugated protein

Amarnadh Nalla, Inge Buch, Birger Hesse

    Publikation: Bidrag til tidsskriftTidsskriftsartikelForskning

    Abstract

    The labelling efficiency of long-term stored DTPA-conjugates has not been reported previously even though DTPA has been in extensive use as metal chelator in the development of radiopharmaceuticals and contrast agents. DTPA is often used as a bifunctional chelating agent conjugated to tumor targeting vehicles such as monoclonal antibodies and receptor directed peptides. The purpose of this study was to monitor the labelling efficiency of a DTPA-conjugate on long-term storage using 111In-chloride at two different temperatures and incubation times for the In-labelling. Method: Cyclic-diethylene-triamine-pentaacetic acid (cDTAP) was conjugated to a polyclonal immunoglobulin-G (IgG) in borate buffer, pH 8.2 at +4oC for 4 hours. Then the DTPA-conjugate was dialyzed against 50 mmol/l sodium citrate buffer saline, pH 6.0 and stored at -80o C in aliquots of 1 mg/0.5 ml. The DTPA-conjugate was labeled with 111In-chloride in citrate buffer, pH 6. The labelling reaction was incubated at room temperature (RT) for 30 min and at +4oC for 90 min. Determi- nation of labelling efficiency was performed using ITLC and an instant chromatography scanner equipped with a NaI crystal. The labelling efficiency of the DTPA-conjugate was monitored every third month for 12 months. Results: The median labelling efficiencies varied between 92 and 96% during the whole period. The two combinations of incubation times and temperatures (30 min at RT and 90 min at +4oC) had no affect on labelling efficiency of the DTPA-conjugate, stored for 12 months. Conclusion: Our study shows that 111In-labelling can easily be performed within 30 min at RT for thermo-stable proteins like polyclonal, DTPA-conjugated IgG stored long-term at -80oC with a high 111In-labelling effi- ciency.
    OriginalsprogEngelsk
    TidsskriftCurrent Radiopharmaceuticals
    Udgave nummer4
    Sider (fra-til)1-4
    Antal sider4
    ISSN1874-4710
    StatusUdgivet - 2011

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