From 2D fluidic array screening to 3D bacterial capturing structures in a point of care system for sepsis diagnosis

Mohammad-Ali Shahbazi; Krishna Kant; Joseph John Kaplinsky; Vinayaka Aaydha Chidambara; Dang Duong Bang; Anders Wolff

doi:10.1109/MEMSYS.2017.7863437

From 2D fluidic array screening to 3D bacterial capturing structures in a point of care system for sepsis diagnosis

Mohammad-Ali Shahbazi
, Krishna Kant
, Joseph John Kaplinsky
, Vinayaka Aaydha Chidambara
, Dang Duong Bang
, Anders Wolff

Danmarks Tekniske Universitet

Publikation: Bidrag til bog/antologi/rapport › Konferenceartikel i proceeding › peer review

Abstract

A combined 2D microfluidic-microarray high throughput approach is reported to identify universal bacterial capturing ligands that can be tethered on the surface of 3D sponges fabricated by different methods for concentrating of bacterial targets in diagnosis devices. The developed platform allows for the first time the simultaneous monitoring of various ligands' affinities to different bacteria species in a dynamic condition in vitro. Moreover, it has been feasible to recognize the effect of steric hindrance on the function of capturing motifs through immobilizing spacer molecules with different lengths between the solid surface and ligands. 3D sponges and micropillars are modified with the most potent capturing molecule to assess their bacterial capturing in real blood samples. Next, the 3D structures are placed into a chip with an immense potential to recognize bacteria through imaging and fluorescence intensity concept.

Originalsprog	Engelsk
Titel	2017 IEEE 30th International Conference on Micro Electro Mechanical Systems, MEMS 2017
Antal sider	4
Forlag	IEEE
Publikationsdato	23 feb. 2017
Sider	440-443
Artikelnummer	7863437
ISBN (Trykt)	978-1-5090-5079-6
ISBN (Elektronisk)	978-1-5090-5078-9
DOI	https://doi.org/10.1109/MEMSYS.2017.7863437
Status	Udgivet - 23 feb. 2017
Udgivet eksternt	Ja
Begivenhed	2017 IEEE 30th International Conference on Micro Electro Mechanical Systems - Las Vegas, USA Varighed: 22 jan. 2017 → 26 jan. 2017 Konferencens nummer: 30 https://ieeexplore.ieee.org/xpl/conhome/7852393/proceeding

Konference

Konference	2017 IEEE 30th International Conference on Micro Electro Mechanical Systems
Nummer	30
Land/Område	USA
By	Las Vegas
Periode	22/01/17 → 26/01/17
Internetadresse	https://ieeexplore.ieee.org/xpl/conhome/7852393/proceeding

Adgang til dokumentet

10.1109/MEMSYS.2017.7863437

Citationsformater

Shahbazi, M.-A., Kant, K., Kaplinsky, J. J., Aaydha Chidambara, V., Bang, D. D., & Wolff, A. (2017). From 2D fluidic array screening to 3D bacterial capturing structures in a point of care system for sepsis diagnosis. I 2017 IEEE 30th International Conference on Micro Electro Mechanical Systems, MEMS 2017 (s. 440-443). Artikel 7863437 IEEE. https://doi.org/10.1109/MEMSYS.2017.7863437

@inproceedings{fde87d4da99e4686bd7f33b10a1922b2,

title = "From 2D fluidic array screening to 3D bacterial capturing structures in a point of care system for sepsis diagnosis",

abstract = "A combined 2D microfluidic-microarray high throughput approach is reported to identify universal bacterial capturing ligands that can be tethered on the surface of 3D sponges fabricated by different methods for concentrating of bacterial targets in diagnosis devices. The developed platform allows for the first time the simultaneous monitoring of various ligands' affinities to different bacteria species in a dynamic condition in vitro. Moreover, it has been feasible to recognize the effect of steric hindrance on the function of capturing motifs through immobilizing spacer molecules with different lengths between the solid surface and ligands. 3D sponges and micropillars are modified with the most potent capturing molecule to assess their bacterial capturing in real blood samples. Next, the 3D structures are placed into a chip with an immense potential to recognize bacteria through imaging and fluorescence intensity concept.",

author = "Mohammad-Ali Shahbazi and Krishna Kant and Kaplinsky, \{Joseph John\} and \{Aaydha Chidambara\}, Vinayaka and Bang, \{Dang Duong\} and Anders Wolff",

note = "Publisher Copyright: {\textcopyright} 2017 IEEE.; 2017 IEEE 30th International Conference on Micro Electro Mechanical Systems, MEMS 2017 ; Conference date: 22-01-2017 Through 26-01-2017",

year = "2017",

month = feb,

day = "23",

doi = "10.1109/MEMSYS.2017.7863437",

language = "English",

isbn = "978-1-5090-5079-6",

pages = "440--443",

booktitle = "2017 IEEE 30th International Conference on Micro Electro Mechanical Systems, MEMS 2017",

publisher = "IEEE",

url = "https://ieeexplore.ieee.org/xpl/conhome/7852393/proceeding",

}

Shahbazi, M-A, Kant, K, Kaplinsky, JJ, Aaydha Chidambara, V, Bang, DD & Wolff, A 2017, From 2D fluidic array screening to 3D bacterial capturing structures in a point of care system for sepsis diagnosis. i 2017 IEEE 30th International Conference on Micro Electro Mechanical Systems, MEMS 2017., 7863437, IEEE, s. 440-443, 2017 IEEE 30th International Conference on Micro Electro Mechanical Systems, Las Vegas, Nevada, USA, 22/01/17. https://doi.org/10.1109/MEMSYS.2017.7863437

From 2D fluidic array screening to 3D bacterial capturing structures in a point of care system for sepsis diagnosis. / Shahbazi, Mohammad-Ali; Kant, Krishna; Kaplinsky, Joseph John et al.
2017 IEEE 30th International Conference on Micro Electro Mechanical Systems, MEMS 2017. IEEE, 2017. s. 440-443 7863437.

Publikation: Bidrag til bog/antologi/rapport › Konferenceartikel i proceeding › peer review

TY - GEN

T1 - From 2D fluidic array screening to 3D bacterial capturing structures in a point of care system for sepsis diagnosis

AU - Shahbazi, Mohammad-Ali

AU - Kant, Krishna

AU - Kaplinsky, Joseph John

AU - Aaydha Chidambara, Vinayaka

AU - Bang, Dang Duong

AU - Wolff, Anders

N1 - Conference code: 30

PY - 2017/2/23

Y1 - 2017/2/23

N2 - A combined 2D microfluidic-microarray high throughput approach is reported to identify universal bacterial capturing ligands that can be tethered on the surface of 3D sponges fabricated by different methods for concentrating of bacterial targets in diagnosis devices. The developed platform allows for the first time the simultaneous monitoring of various ligands' affinities to different bacteria species in a dynamic condition in vitro. Moreover, it has been feasible to recognize the effect of steric hindrance on the function of capturing motifs through immobilizing spacer molecules with different lengths between the solid surface and ligands. 3D sponges and micropillars are modified with the most potent capturing molecule to assess their bacterial capturing in real blood samples. Next, the 3D structures are placed into a chip with an immense potential to recognize bacteria through imaging and fluorescence intensity concept.

AB - A combined 2D microfluidic-microarray high throughput approach is reported to identify universal bacterial capturing ligands that can be tethered on the surface of 3D sponges fabricated by different methods for concentrating of bacterial targets in diagnosis devices. The developed platform allows for the first time the simultaneous monitoring of various ligands' affinities to different bacteria species in a dynamic condition in vitro. Moreover, it has been feasible to recognize the effect of steric hindrance on the function of capturing motifs through immobilizing spacer molecules with different lengths between the solid surface and ligands. 3D sponges and micropillars are modified with the most potent capturing molecule to assess their bacterial capturing in real blood samples. Next, the 3D structures are placed into a chip with an immense potential to recognize bacteria through imaging and fluorescence intensity concept.

U2 - 10.1109/MEMSYS.2017.7863437

DO - 10.1109/MEMSYS.2017.7863437

M3 - Conference contribution to proceeding

SN - 978-1-5090-5079-6

SP - 440

EP - 443

BT - 2017 IEEE 30th International Conference on Micro Electro Mechanical Systems, MEMS 2017

PB - IEEE

T2 - 2017 IEEE 30th International Conference on Micro Electro Mechanical Systems

Y2 - 22 January 2017 through 26 January 2017

ER -

From 2D fluidic array screening to 3D bacterial capturing structures in a point of care system for sepsis diagnosis

Abstract

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