Human anti-rhesus D IgG1 antibody produced in transgenic plants

Thomas Bouquin, Mads Thomsen, Leif Kofoed Nielsen, Trine Hefsgaard Green, John Mundy, Morten Hanefeld Dziegiel

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Transgenic plants represent an alternative to cell culture systems for producing cheap and safe antibodies for diagnostic and therapeutic use. To evaluate the functional properties of a 'plantibody', we generated transgenic Arabidopsis plants expressing full-length human IgG1 against the Rhesus D antigen, which is responsible for alloimmunization of RhD- mothers carrying an RhD+ fetus. Anti-RhD extracted from plants specifically reacted with RhD+ cells in antiglobulin technique, and elicited a respiratory burst in human peripheral blood mononuclear cells. Plant-derived antibody had equivalent properties to CHO cell-produced anti-RhD antibody, indicating its potential usefulness in diagnostic and therapeutic programs.

TidsskriftTransgenic Research
Udgave nummer2
Sider (fra-til)115-22
Antal sider8
StatusUdgivet - apr. 2002
Udgivet eksterntJa