Human anti-rhesus D IgG1 antibody produced in transgenic plants

Thomas Bouquin, Mads Thomsen, Leif Kofoed Nielsen, Trine Hefsgaard Green, John Mundy, Morten Hanefeld Dziegiel

Publikation: Bidrag til tidsskriftTidsskriftsartikelForskningpeer review

Abstract

Transgenic plants represent an alternative to cell culture systems for producing cheap and safe antibodies for diagnostic and therapeutic use. To evaluate the functional properties of a 'plantibody', we generated transgenic Arabidopsis plants expressing full-length human IgG1 against the Rhesus D antigen, which is responsible for alloimmunization of RhD- mothers carrying an RhD+ fetus. Anti-RhD extracted from plants specifically reacted with RhD+ cells in antiglobulin technique, and elicited a respiratory burst in human peripheral blood mononuclear cells. Plant-derived antibody had equivalent properties to CHO cell-produced anti-RhD antibody, indicating its potential usefulness in diagnostic and therapeutic programs.

OriginalsprogEngelsk
TidsskriftTransgenic Research
Vol/bind11
Udgave nummer2
Sider (fra-til)115-22
Antal sider8
ISSN0962-8819
StatusUdgivet - apr. 2002
Udgivet eksterntJa

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