Methods for Isolation, Purification, and Propagation of Bacteriophages of Campylobacter jejuni

Yilmaz Emre Gencay, Tina Birk, Martine Camilla Holst Sørensen, Lone Brøndsted

Publikation: Bidrag til bog/antologi/rapportBidrag til bog/antologiForskningpeer review

Abstract

Here, we describe the methods for isolation, purification, and propagation of Campylobacter jejuni bacteriophages from samples expected to contain high number of phages such as chicken feces. The overall steps are (1) liberation of phages from the sample material; (2) observation of plaque-forming units on C. jejuni lawns using a spot assay; (3) isolation of single plaques; (4) consecutive purification procedures; and (5) propagation of purified phages from a plate lysate to prepare master stocks.
OriginalsprogEngelsk
TitelCampylobacter jejuni - methods and protocols
RedaktørerJames Butcher, Alain Stintzi
Antal sider10
ForlagSpringer Science+Business Media
Publikationsdato2017
Sider19-28
ISBN (Trykt)978-1-4939-6534-2
DOI
StatusUdgivet - 2017
Udgivet eksterntJa
NavnMethods in Molecular Biology
ISSN1064-3745

Emneord

  • Bacteriophage
  • C. jejuni
  • Campylobacter
  • Isolation
  • Propagation
  • Purification
  • Titration

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