Microbial Degradation of Complex Organic Compounds in a Danish Drinking Water Pipeline Distribution System

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Abstract

In this pilot study, biofilms found in the Danish water distribution system were
investigated for their ability to biodegrade three specific compounds that were
found to leach from PE pipes into the drinking water distribution system.
• The compounds tested were dissolved in methanol and were as follows: 7,9-ditert-Butyl-1-oxaspiro(4,5)deca-6,9-diene-2,8-dione (40 ng/ml final conc); 2,4-ditert-butylphenol (100 ng/ml final conc) and 3-(3,5-di-tert-butyl-4-hydroxyphenyl)
propionic acid (100 ng/ml final conc). Cocktail Mix (1:1:1 mixture of the above).
• Two biofilm sample types were studied: PE pipe biofilm samples collected in
proximity to consumers (BUS), and PE pipe biofilm samples collected close to
the groundwater source (TBR). The photos below show the biofilm rigs located
in the water works near the ground water source.
• Enrichment cultures were set up with each of the biofilm sample types
incubated in minimal salts medium containing the PE pipe leached substrates
as the sole carbon and energy source. Cultures were incubated in the dark
with shaking (110 rpm) for 73 days at 12ºC. Abiotic controls were also prepared
without any biofilm inoculum, to determine whether any abiotic losses had
occurred. Biodegradation was monitored by GC-MS analysis.
• Changes in the bacterial and archaeal communities during biodegradation
were also quantified by qPCR analysis of the 16S rRNA genes.
OriginalsprogEngelsk
Publikationsdato5 dec. 2022
StatusUdgivet - 5 dec. 2022
BegivenhedIWA Biofilms 2022
- Phuket, Thailand
Varighed: 6 dec. 20228 dec. 2022
https://iwabiofilms2020.org/

Konference

KonferenceIWA Biofilms 2022
Land/OmrådeThailand
ByPhuket
Periode06/12/2208/12/22
Internetadresse

Emneord

  • Byggeri, miljø og energi

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