TY - JOUR
T1 - Phosphoribosylpyrophosphate (PRPP)-less mutants of Escherichia coli
AU - Hove-Jensen, Bjarne
PY - 1989/11
Y1 - 1989/11
N2 - A DNA fragment encoding kanamycin resistance was inserted in vitro into a plasmid‐borne prs gene encoding phosphoribosylpyrophosphate synthetase of Escherichia coli. The resulting plasmids were subsequently transferred to the chromosome by homologous recombination and the haploid strains prs‐3::Kan R and prs‐4::Kan R were obtained. These strains were fully viable, but required guanosine, uridine, histidine, tryptophan and nicotinamide mononucleotide. There was no phosphoribosylpyrophosphate synthetase activity or phosphoribosylpyrophosphate pool in the mutant strains. These results show that phosphoribosytpyrophosphate synthetase is dispensable for E. coli.
AB - A DNA fragment encoding kanamycin resistance was inserted in vitro into a plasmid‐borne prs gene encoding phosphoribosylpyrophosphate synthetase of Escherichia coli. The resulting plasmids were subsequently transferred to the chromosome by homologous recombination and the haploid strains prs‐3::Kan R and prs‐4::Kan R were obtained. These strains were fully viable, but required guanosine, uridine, histidine, tryptophan and nicotinamide mononucleotide. There was no phosphoribosylpyrophosphate synthetase activity or phosphoribosylpyrophosphate pool in the mutant strains. These results show that phosphoribosytpyrophosphate synthetase is dispensable for E. coli.
UR - https://www.scopus.com/pages/publications/0024368029
U2 - 10.1111/j.1365-2958.1989.tb00134.x
DO - 10.1111/j.1365-2958.1989.tb00134.x
M3 - Journal article
SN - 0950-382X
VL - 3
SP - 1487
EP - 1492
JO - Molecular Microbiology
JF - Molecular Microbiology
IS - 11
ER -