TY - JOUR
T1 - Secreted protein acidic and rich in cysteine (SPARC) in human skeletal muscle
AU - Jørgensen, Louise Helskov
AU - Petersson, Stine Juhl
AU - Sellathurai, Jeeva
AU - Andersen, Ditte C.
AU - Thayssen, Susanne
AU - Sant, Dorte J
AU - Jensen, Charlotte Harken
AU - Schrøder, Henrik Daa
PY - 2009/1
Y1 - 2009/1
N2 - Secreted protein acidic and rich in cysteine (SPARC)/osteonectin is expressed in different tissues during remodeling and repair, suggesting a function in regeneration. Several gene expression studies indicated that SPARC was expressed in response to muscle damage. Studies on myoblasts further indicated a function of SPARC in skeletal muscle. We therefore found it of interest to study SPARC expression in human skeletal muscle during development and in biopsies from Duchenne and Becker muscular dystrophy and congenital muscular dystrophy, congenital myopathy, inclusion body myositis, and polymyositis patients to analyze SPARC expression in a selected range of inherited and idiopathic muscle wasting diseases. SPARC-positive cells were observed both in fetal and neonatal muscle, and in addition, fetal myofibers were observed to express SPARC at the age of 15-16 weeks. SPARC protein was detected in the majority of analyzed muscle biopsies (23 of 24), mainly in mononuclear cells of which few were pax7 positive. Myotubes and regenerating myofibers also expressed SPARC. The expression-degree seemed to reflect the severity of the lesion. In accordance with these in vivo findings, primary human-derived satellite cells were found to express SPARC both during proliferation and differentiation in vitro. In conclusion, this study shows SPARC expression both during muscle development and in regenerating muscle. The expression is detected both in satellite cells/myoblasts and in myotubes and muscle fibers, indicating a role for SPARC in the skeletal muscle compartment.
AB - Secreted protein acidic and rich in cysteine (SPARC)/osteonectin is expressed in different tissues during remodeling and repair, suggesting a function in regeneration. Several gene expression studies indicated that SPARC was expressed in response to muscle damage. Studies on myoblasts further indicated a function of SPARC in skeletal muscle. We therefore found it of interest to study SPARC expression in human skeletal muscle during development and in biopsies from Duchenne and Becker muscular dystrophy and congenital muscular dystrophy, congenital myopathy, inclusion body myositis, and polymyositis patients to analyze SPARC expression in a selected range of inherited and idiopathic muscle wasting diseases. SPARC-positive cells were observed both in fetal and neonatal muscle, and in addition, fetal myofibers were observed to express SPARC at the age of 15-16 weeks. SPARC protein was detected in the majority of analyzed muscle biopsies (23 of 24), mainly in mononuclear cells of which few were pax7 positive. Myotubes and regenerating myofibers also expressed SPARC. The expression-degree seemed to reflect the severity of the lesion. In accordance with these in vivo findings, primary human-derived satellite cells were found to express SPARC both during proliferation and differentiation in vitro. In conclusion, this study shows SPARC expression both during muscle development and in regenerating muscle. The expression is detected both in satellite cells/myoblasts and in myotubes and muscle fibers, indicating a role for SPARC in the skeletal muscle compartment.
KW - Blotting, Western
KW - Cell Differentiation
KW - Humans
KW - Immunohistochemistry
KW - Infant, Newborn
KW - Journal Article
KW - Muscle, Skeletal
KW - Muscular Diseases
KW - Muscular Dystrophies
KW - Myositis, Inclusion Body
KW - Osteonectin
KW - Polymyositis
KW - Research Support, Non-U.S. Gov't
KW - Reverse Transcriptase Polymerase Chain Reaction
KW - Satellite Cells, Skeletal Muscle
U2 - 10.1369/jhc.2008.951954
DO - 10.1369/jhc.2008.951954
M3 - Journal article
C2 - 18796407
SN - 0022-1554
VL - 57
SP - 29
EP - 39
JO - Journal of Histochemistry and Cytochemistry
JF - Journal of Histochemistry and Cytochemistry
IS - 1
ER -