Skeletal muscle fiber characteristics and oxidative capacity in hemiparetic stroke survivors

Kaare Severinsen, Ulrik Dalgas, Kristian Overgaard, Asger R Pedersen, Niels Ørtenblad, Caroline Lund, Johannes K Jakobsen, Henning Andersen

Publikation: Bidrag til tidsskriftTidsskriftsartikelForskningpeer review

Abstract

INTRODUCTION: Skeletal muscle is changed after stroke, but conflicting data exist concerning muscle morphology and oxidative enzyme capacity.

METHODS: In 36 chronic stroke patients bilateral rectus femoris muscle biopsies were analyzed, and fiber type proportions and cross-sectional areas were determined by ATPase histochemistry. Enzymatic concentrations of citrate synthase (CS) and 3-Hydroxyacyl-coenzymeA-dehydrogenase (HAD) were determined using freeze-dried muscle tissue. Findings were correlated with clinical outcomes.

RESULTS: In the paretic muscles the mean fiber area was smaller (P = 0.0004), and a lower proportion of type 1 fibers (P = 0.0016) and a higher proportion of type 2X fibers (P = 0.0002) were observed. The paretic muscle had lower CS (P = 0.013) and HAD concentrations (P = 0.037). Mean fiber area correlated with muscle strength (r = 0.43; P = 0.041), and CS concentration correlated with aerobic capacity (r = 0.47; P = 0.01).

CONCLUSIONS: In stroke survivors there is a phenotypic shift toward more fatigable muscle fibers with reduced oxidative enzymatic capacity that relates to clinical outcomes.

OriginalsprogEngelsk
TidsskriftMuscle & Nerve
Vol/bind53
Udgave nummer5
Sider (fra-til)748-54
Antal sider7
ISSN0148-639X
DOI
StatusUdgivet - maj 2016

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