TY - JOUR
T1 - YKL-40 tissue expression and plasma levels in patients with ovarian cancer
AU - Høgdall, Estrid V.S.
AU - Ringsholt, Merete
AU - Christensen, Ib Jarle
AU - Johansen, Julia S.
AU - Kjær, Susanne K.
AU - Blaakaer, Jan
AU - Ostenfeld-Møller, Lene
AU - Price, Paul A.
AU - Christensen, Lise H.
N1 - Funding Information: We thank all nurses and doctors on the gynecological and pathological departments for their tremendous work. The authors are grateful to Heidi Marie Paulsen (Rigshopitalet), Vibeke Reese (Danish State Serum Institute), Tonni Løve Hansen, and Debbie Nadelmann (Herlev Hospital) for technical assistance. This work was supported by grants from Mermaid1, The Danish Cancer Society and National Cancer Institute, Bethesda, USA (RO1 CA 61107), "Direktør Jens Aage Sørensen og Hustru Edith Ingeborg Sørensens Mindefond" and "Vera og Carl Johan Michaelsens legat". Quidel provided the study with YKL-40 ELISA kits.
PY - 2009/1/9
Y1 - 2009/1/9
N2 - Background: YKL-40 (chitinase-3-like-1) is a member of "mammalian chitinase-like proteins". The protein is expressed in many types of cancer cells and the highest plasma YKL-40 levels have been found in patients with metastatic disease, short recurrence/progression-free intervals, and short overall survival. The aim of the study was to determine the expression of YKL-40 in tumor tissue and plasma in patients with borderline ovarian tumor or epithelial ovarian cancer (OC), and investigate prognostic value of this marker. Methods: YKL-40 protein expression was determined by immunohistochemistry in tissue arrays from 181 borderline tumors and 473 OC. Plasma YKL-40 was determined by ELISA in preoperative samples from 19 patients with borderline tumor and 76 OC patients. Results: YKL-40 protein expression was found in cancer cells, tumor associated macrophages, neutrophils and mast cells. The tumor cell expression was higher in OC than in borderline tumors (p = 0.001), and associated with FIGO stage (p < 0.0001) and histological subtype (p = 0.0009). Positive YKL-40 expression (≥ 5% staining) was not associated with reduced survival. Plasma YKL-40 was also higher in patients with OC than in patients with borderline tumors (p < 0.0001), and it was positively correlated to serum CA-125 (p < 0.0001) and FIGO stage (p = 0.0001). Univariate Cox analysis of plasma YKL-40 showed association with overall survival (p < 0.0001). Multivariate Cox analysis, including plasma YKL-40, serum CA125, FIGO stage, age and radicality after primary surgery as variables, showed that elevated plasma YKL-40 was associated with a shorter survival (HR = 2.13, 95% CI: 1.40-3.25, p = 0.0004). Conclusion: YKL-40 in OC tissue and plasma are related to stage and histology, but only plasma YKL-40 is a prognostic biomarker in patients with OC.
AB - Background: YKL-40 (chitinase-3-like-1) is a member of "mammalian chitinase-like proteins". The protein is expressed in many types of cancer cells and the highest plasma YKL-40 levels have been found in patients with metastatic disease, short recurrence/progression-free intervals, and short overall survival. The aim of the study was to determine the expression of YKL-40 in tumor tissue and plasma in patients with borderline ovarian tumor or epithelial ovarian cancer (OC), and investigate prognostic value of this marker. Methods: YKL-40 protein expression was determined by immunohistochemistry in tissue arrays from 181 borderline tumors and 473 OC. Plasma YKL-40 was determined by ELISA in preoperative samples from 19 patients with borderline tumor and 76 OC patients. Results: YKL-40 protein expression was found in cancer cells, tumor associated macrophages, neutrophils and mast cells. The tumor cell expression was higher in OC than in borderline tumors (p = 0.001), and associated with FIGO stage (p < 0.0001) and histological subtype (p = 0.0009). Positive YKL-40 expression (≥ 5% staining) was not associated with reduced survival. Plasma YKL-40 was also higher in patients with OC than in patients with borderline tumors (p < 0.0001), and it was positively correlated to serum CA-125 (p < 0.0001) and FIGO stage (p = 0.0001). Univariate Cox analysis of plasma YKL-40 showed association with overall survival (p < 0.0001). Multivariate Cox analysis, including plasma YKL-40, serum CA125, FIGO stage, age and radicality after primary surgery as variables, showed that elevated plasma YKL-40 was associated with a shorter survival (HR = 2.13, 95% CI: 1.40-3.25, p = 0.0004). Conclusion: YKL-40 in OC tissue and plasma are related to stage and histology, but only plasma YKL-40 is a prognostic biomarker in patients with OC.
UR - http://www.scopus.com/inward/record.url?scp=61849164601&partnerID=8YFLogxK
U2 - 10.1186/1471-2407-9-8
DO - 10.1186/1471-2407-9-8
M3 - Journal article
SN - 1471-2407
VL - 9
JO - BMC Cancer
JF - BMC Cancer
IS - 8
M1 - 8
ER -