A Disposable Polymer Lab-On-A-Slide For Point-Of-Care Diagnostics Of Methicillin-Resistant Staphylococcus Aureus (Mrsa)

Minqiang Bu; Ivan R. Perch-Nielsen; Julia Skov; Karen Skotte Sørensen; Sun Yi; Michael E. Pedersen; Mikkel Fougt Hansen; Kenneth W. Harlow; Anders Wolff

doi:10.1109/Transducers.2013.6627214

A Disposable Polymer Lab-On-A-Slide For Point-Of-Care Diagnostics Of Methicillin-Resistant Staphylococcus Aureus (Mrsa)

Minqiang Bu
, Ivan R. Perch-Nielsen
, Julia Skov
, Karen Skotte Sørensen
, Sun Yi
, Michael E. Pedersen
, Mikkel Fougt Hansen
, Kenneth W. Harlow
, Anders Wolff

Technical University of Denmark

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution to proceeding › peer-review

Abstract

This paper reports the design, fabrication and experimental verification of a polymer microfluidic labon-a-slide for rapid detection of methicillin-resistant Staphylococcus aureus (MRSA). MRSA cells were captured in a lysis chamber using magnetic beads, followed by thermal lysis. The released DNA was transferred into a second chamber for polymerase chain reaction (PCR) amplification. Fluidic control in the device was accomplished by pneumatic actuation of a micropump and five microvalves integrated on the device. The mecA gene from MRSA was successfully amplified by real-time PCR within 35 min. Presence of the correct PCR amplicon (186 bp) was verified using capillary electrophoresis. The results demonstrate the utility of a low-cost disposable polymer microfluidic system for rapid pathogen detection for point-of-care diagnostics.

Original language	English
Title of host publication	2013 Transducers and Eurosensors XXVII : The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013
Number of pages	4
Publisher	IEEE
Publication date	2013
Pages	2098-2101
Article number	6627214
ISBN (Print)	9781467359818
DOIs	https://doi.org/10.1109/Transducers.2013.6627214
Publication status	Published - 2013
Externally published	Yes
Event	17th International Conference on Solid-State Sensors, Actuators and Microsystems - Centro de Convenciones Internacional de Barcelona (CCIB), Barcelona, Spain Duration: 16 Jun 2013 → 20 Jun 2013 Conference number: 17 http://transducers-eurosensors2013.org/

Conference

Conference	17th International Conference on Solid-State Sensors, Actuators and Microsystems
Number	17
Location	Centro de Convenciones Internacional de Barcelona (CCIB)
Country/Territory	Spain
City	Barcelona
Period	16/06/13 → 20/06/13
Internet address	http://transducers-eurosensors2013.org/

Keywords

MRSA detection
Microfluidics
PCR amplification
cell lysis
lab-on-a-slide
point-of-care diagnostics

Access to Document

10.1109/Transducers.2013.6627214

Full textSubmitted manuscript, 890 KB

Cite this

Bu, M., R. Perch-Nielsen, I., Skov, J., Sørensen, K. S., Yi, S., E. Pedersen, M., Hansen, M. F., W. Harlow, K., & Wolff, A. (2013). A Disposable Polymer Lab-On-A-Slide For Point-Of-Care Diagnostics Of Methicillin-Resistant Staphylococcus Aureus (Mrsa). In 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013 (pp. 2098-2101). Article 6627214 IEEE. https://doi.org/10.1109/Transducers.2013.6627214

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title = "A Disposable Polymer Lab-On-A-Slide For Point-Of-Care Diagnostics Of Methicillin-Resistant Staphylococcus Aureus (Mrsa)",

abstract = "This paper reports the design, fabrication and experimental verification of a polymer microfluidic labon-a-slide for rapid detection of methicillin-resistant Staphylococcus aureus (MRSA). MRSA cells were captured in a lysis chamber using magnetic beads, followed by thermal lysis. The released DNA was transferred into a second chamber for polymerase chain reaction (PCR) amplification. Fluidic control in the device was accomplished by pneumatic actuation of a micropump and five microvalves integrated on the device. The mecA gene from MRSA was successfully amplified by real-time PCR within 35 min. Presence of the correct PCR amplicon (186 bp) was verified using capillary electrophoresis. The results demonstrate the utility of a low-cost disposable polymer microfluidic system for rapid pathogen detection for point-of-care diagnostics.",

keywords = "MRSA detection, Microfluidics, PCR amplification, cell lysis, lab-on-a-slide, point-of-care diagnostics",

author = "Minqiang Bu and \{R. Perch-Nielsen\}, Ivan and Julia Skov and S{\o}rensen, \{Karen Skotte\} and Sun Yi and \{E. Pedersen\}, Michael and Hansen, \{Mikkel Fougt\} and \{W. Harlow\}, Kenneth and Anders Wolff",

year = "2013",

doi = "10.1109/Transducers.2013.6627214",

language = "English",

isbn = "9781467359818",

pages = "2098--2101",

booktitle = "2013 Transducers and Eurosensors XXVII",

publisher = "IEEE",

note = "17th International Conference on Solid-State Sensors, Actuators and Microsystems ; Conference date: 16-06-2013 Through 20-06-2013",

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Bu, M, R. Perch-Nielsen, I, Skov, J, Sørensen, KS, Yi, S, E. Pedersen, M, Hansen, MF, W. Harlow, K & Wolff, A 2013, A Disposable Polymer Lab-On-A-Slide For Point-Of-Care Diagnostics Of Methicillin-Resistant Staphylococcus Aureus (Mrsa). in 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013., 6627214, IEEE, pp. 2098-2101, 17th International Conference on Solid-State Sensors, Actuators and Microsystems, Barcelona, Spain, 16/06/13. https://doi.org/10.1109/Transducers.2013.6627214

A Disposable Polymer Lab-On-A-Slide For Point-Of-Care Diagnostics Of Methicillin-Resistant Staphylococcus Aureus (Mrsa). / Bu, Minqiang; R. Perch-Nielsen, Ivan; Skov, Julia et al.
2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013. IEEE, 2013. p. 2098-2101 6627214.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution to proceeding › peer-review

TY - GEN

T1 - A Disposable Polymer Lab-On-A-Slide For Point-Of-Care Diagnostics Of Methicillin-Resistant Staphylococcus Aureus (Mrsa)

AU - Bu, Minqiang

AU - R. Perch-Nielsen, Ivan

AU - Skov, Julia

AU - Sørensen, Karen Skotte

AU - Yi, Sun

AU - E. Pedersen, Michael

AU - Hansen, Mikkel Fougt

AU - W. Harlow, Kenneth

AU - Wolff, Anders

N1 - Conference code: 17

PY - 2013

Y1 - 2013

N2 - This paper reports the design, fabrication and experimental verification of a polymer microfluidic labon-a-slide for rapid detection of methicillin-resistant Staphylococcus aureus (MRSA). MRSA cells were captured in a lysis chamber using magnetic beads, followed by thermal lysis. The released DNA was transferred into a second chamber for polymerase chain reaction (PCR) amplification. Fluidic control in the device was accomplished by pneumatic actuation of a micropump and five microvalves integrated on the device. The mecA gene from MRSA was successfully amplified by real-time PCR within 35 min. Presence of the correct PCR amplicon (186 bp) was verified using capillary electrophoresis. The results demonstrate the utility of a low-cost disposable polymer microfluidic system for rapid pathogen detection for point-of-care diagnostics.

AB - This paper reports the design, fabrication and experimental verification of a polymer microfluidic labon-a-slide for rapid detection of methicillin-resistant Staphylococcus aureus (MRSA). MRSA cells were captured in a lysis chamber using magnetic beads, followed by thermal lysis. The released DNA was transferred into a second chamber for polymerase chain reaction (PCR) amplification. Fluidic control in the device was accomplished by pneumatic actuation of a micropump and five microvalves integrated on the device. The mecA gene from MRSA was successfully amplified by real-time PCR within 35 min. Presence of the correct PCR amplicon (186 bp) was verified using capillary electrophoresis. The results demonstrate the utility of a low-cost disposable polymer microfluidic system for rapid pathogen detection for point-of-care diagnostics.

KW - MRSA detection

KW - Microfluidics

KW - PCR amplification

KW - cell lysis

KW - lab-on-a-slide

KW - point-of-care diagnostics

U2 - 10.1109/Transducers.2013.6627214

DO - 10.1109/Transducers.2013.6627214

M3 - Conference contribution to proceeding

SN - 9781467359818

SP - 2098

EP - 2101

BT - 2013 Transducers and Eurosensors XXVII

PB - IEEE

T2 - 17th International Conference on Solid-State Sensors, Actuators and Microsystems

Y2 - 16 June 2013 through 20 June 2013

ER -

Bu M, R. Perch-Nielsen I, Skov J, Sørensen KS, Yi S, E. Pedersen M et al. A Disposable Polymer Lab-On-A-Slide For Point-Of-Care Diagnostics Of Methicillin-Resistant Staphylococcus Aureus (Mrsa). In 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013. IEEE. 2013. p. 2098-2101. 6627214 doi: 10.1109/Transducers.2013.6627214

A Disposable Polymer Lab-On-A-Slide For Point-Of-Care Diagnostics Of Methicillin-Resistant Staphylococcus Aureus (Mrsa)

Abstract

Conference

Keywords

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