Assessments of individual fiber glycogen and mitochondrial volume percentages reveal a graded reduction in muscle oxidative power during prolonged exhaustive exercise

During submaximal exercise, there is a heterogeneous recruitment of skeletal muscle fibers, with an ensuing heterogeneous depletion of muscle glycogen both within and between fiber types. Here, we show that the mean (95% CI) mitochon-drial volume as a percentage of fiber volume of non- glycogen- depleted fibers was 2 (−10:6), 5 (−21:11), and 12 (−21:−2)% lower than all the sampled fibers after continuing exercise for 1, 2 h, and until task failure, respectively. Therefore, a glycogen- dependent fatigue of individual fibers during submaximal exercise may reduce the muscular oxidative power. These findings suggest a relationship be-tween glycogen and mitochondrial content in individual muscle fibers, which is important for understanding fatigue during prolonged exercise