Chemically modified oligonucleotides with efficient RNase H response

Birte Vester; Anne Marie Boel; Sune Lobedanz; B Ravindra Babu; Michael Raunkjaer; Dorthe Lindegaard; null Raunak; Patrick J Hrdlicka; Torben René Højland; Pawan K Sharma; Surender Kumar; Jesper Wengel; Poul Nielsen

doi:10.1016/j.bmcl.2008.03.004

Chemically modified oligonucleotides with efficient RNase H response

Birte Vester, Anne Marie Boel, Sune Lobedanz, B Ravindra Babu, Michael Raunkjaer, Dorthe Lindegaard, Raunak, Patrick J Hrdlicka, Torben René Højland, Pawan K Sharma, Surender Kumar, Jesper Wengel, Poul Nielsen

University of Southern Denmark

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

Ten different chemically modified nucleosides were incorporated into short DNA strands (chimeric oligonucleotides ON3-ON12 and ON15-ON24) and then tested for their capacity to mediate RNAse H cleavage of the complementary RNA strand. The modifications were placed at two central positions directly in the RNase H cleaving region. The RNA strand of duplexes with ON3, ON5 and ON12 were cleaved more efficiently than the RNA strand of the DNA:RNA control duplex. There seems to be no correlation between the thermal stability between the duplexes and RNase H cleavage.

Original language	English
Journal	Bioorganic & Medicinal Chemistry Letters
ISSN	0960-894X
DOIs	https://doi.org/10.1016/j.bmcl.2008.03.004
Publication status	Published - 1 Apr 2008
Externally published	Yes

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10.1016/j.bmcl.2008.03.004

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@article{c3003dcec4534e9d90d4f7e4ea2fc522,

title = "Chemically modified oligonucleotides with efficient RNase H response",

abstract = "Ten different chemically modified nucleosides were incorporated into short DNA strands (chimeric oligonucleotides ON3-ON12 and ON15-ON24) and then tested for their capacity to mediate RNAse H cleavage of the complementary RNA strand. The modifications were placed at two central positions directly in the RNase H cleaving region. The RNA strand of duplexes with ON3, ON5 and ON12 were cleaved more efficiently than the RNA strand of the DNA:RNA control duplex. There seems to be no correlation between the thermal stability between the duplexes and RNase H cleavage.",

author = "Birte Vester and Boel, {Anne Marie} and Sune Lobedanz and Babu, {B Ravindra} and Michael Raunkjaer and Dorthe Lindegaard and Raunak and Hrdlicka, {Patrick J} and H{\o}jland, {Torben Ren{\'e}} and Sharma, {Pawan K} and Surender Kumar and Jesper Wengel and Poul Nielsen",

year = "2008",

month = apr,

day = "1",

doi = "10.1016/j.bmcl.2008.03.004",

language = "English",

journal = "Bioorganic & Medicinal Chemistry Letters",

issn = "0960-894X",

publisher = "Elsevier",

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TY - JOUR

T1 - Chemically modified oligonucleotides with efficient RNase H response

AU - Vester, Birte

AU - Boel, Anne Marie

AU - Lobedanz, Sune

AU - Babu, B Ravindra

AU - Raunkjaer, Michael

AU - Lindegaard, Dorthe

AU - Raunak, null

AU - Hrdlicka, Patrick J

AU - Højland, Torben René

AU - Sharma, Pawan K

AU - Kumar, Surender

AU - Wengel, Jesper

AU - Nielsen, Poul

PY - 2008/4/1

Y1 - 2008/4/1

N2 - Ten different chemically modified nucleosides were incorporated into short DNA strands (chimeric oligonucleotides ON3-ON12 and ON15-ON24) and then tested for their capacity to mediate RNAse H cleavage of the complementary RNA strand. The modifications were placed at two central positions directly in the RNase H cleaving region. The RNA strand of duplexes with ON3, ON5 and ON12 were cleaved more efficiently than the RNA strand of the DNA:RNA control duplex. There seems to be no correlation between the thermal stability between the duplexes and RNase H cleavage.

AB - Ten different chemically modified nucleosides were incorporated into short DNA strands (chimeric oligonucleotides ON3-ON12 and ON15-ON24) and then tested for their capacity to mediate RNAse H cleavage of the complementary RNA strand. The modifications were placed at two central positions directly in the RNase H cleaving region. The RNA strand of duplexes with ON3, ON5 and ON12 were cleaved more efficiently than the RNA strand of the DNA:RNA control duplex. There seems to be no correlation between the thermal stability between the duplexes and RNase H cleavage.

UR - https://portal.findresearcher.sdu.dk/en/publications/f04a5030-4e5d-11dd-b1a1-000ea68e967b

U2 - 10.1016/j.bmcl.2008.03.004

DO - 10.1016/j.bmcl.2008.03.004

M3 - Journal article

C2 - 18356048

SN - 0960-894X

JO - Bioorganic & Medicinal Chemistry Letters

JF - Bioorganic & Medicinal Chemistry Letters

ER -

Chemically modified oligonucleotides with efficient RNase H response

Abstract

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