Projects per year
Water-based extracts (Fig. 1b) were produced from four different halophyte species in the laboratory. A series of anaerobic flasks with Postgate B Medium were inoculated with a mixed anaerobic microbial culture. Each flask was treated with one of the extracts at four concentrations (5, 10, 15, and 20% v/v). At 20%, ATP and H2S measurements showed that adding the extract resulted in decreased ATP concentrations and no H2S production. This indicated a selective inhibition of sulfate-reducing bacteria (SRB). Subsequent 16S rRNA amplicon sequencing, measurements of coupon weight loss, and 3D-topography of coupons have confirmed this hypothesis. The sequencing data showed that the relative abundance of SRB decreased below the detectable limits. Only a few species, which have not been linked to MIC, were able to survive the treatment. Subsequent tests of the extract in an anaerobic, closed-loop CDC Biofilm Reactor showed a capability to break apart an established anaerobic biofilm.
This study used the multiple lines of evidence (MLOE) approach to demonstrate that halophyte-based extracts have potential as a mitigation strategy for MIC. However, these crude plant extracts are chemically complex. This currently poses problems with legislation as the entire chemical composition is not strictly defined and may vary between batches.
|Publication date||26 Aug 2022|
|Publication status||Published - 26 Aug 2022|
|Event||COST Conference: Microbiologically influenced Corrosion - BAM, Berlin, Germany|
Duration: 26 Aug 2022 → 26 Aug 2022
|Conference||COST Conference: Microbiologically influenced Corrosion|
|Period||26/08/22 → 26/08/22|
FingerprintDive into the research topics of 'Clean Biocide Project: Halophyte Extracts as Natural Corrosion Inhibitors in Water Systems'. Together they form a unique fingerprint.
Skovhus, T. L., Thomsen, M. H., Chaturvedi, T. & Stein, J. L.
01/11/21 → 31/12/23
Clean Biocide Phase 1: Halophilic plant extracts as natural corrosion inhibitors and biocides for oil field application
Skovhus, T. L., Thomsen, M. H., Chaturvedi, T., Eckert, R., Stein, J. L. & Kotu, S. P.
01/09/20 → 01/09/21