D-Allose catabolism of Escherichia coli: Involvement of alsI and regulation of als regulon expression by allose and ribose

Tim S. Poulsen; Ying-Ying Chang; Bjarne Hove-Jensen

doi:10.1128/jb.181.22.7126-7130.1999

D-Allose catabolism of Escherichia coli: Involvement of alsI and regulation of als regulon expression by allose and ribose

Tim S. Poulsen
, Ying-Ying Chang
, Bjarne Hove-Jensen

University of Copenhagen

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

Genes involved in allose utilization of Escherichia coli K-12 are organized in at least two operons, alsRBACE and alsI, located next to each other on the chromosome but divergently transcribed. Mutants defective in alsI (allose 6-phosphate isomerase gene) and alsE (allulose 6-phosphate epimerase gene) were Als ^-. Transcription of the two allose operons, measured as β-galactosidase activity specified by alsI-lacZ ⁺ or alsE-lacZ ⁺ operon fusions, was induced by allose. Ribose also caused derepression of expression of the regulon under conditions in which ribose phosphate catabolism was impaired.

Original language	English
Journal	Journal of Bacteriology
Volume	181
Issue number	22
Pages (from-to)	7126-7130
Number of pages	5
ISSN	0021-9193
DOIs	https://doi.org/10.1128/jb.181.22.7126-7130.1999
Publication status	Published - Nov 1999

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10.1128/jb.181.22.7126-7130.1999

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title = "D-Allose catabolism of Escherichia coli: Involvement of alsI and regulation of als regulon expression by allose and ribose",

abstract = "Genes involved in allose utilization of Escherichia coli K-12 are organized in at least two operons, alsRBACE and alsI, located next to each other on the chromosome but divergently transcribed. Mutants defective in alsI (allose 6-phosphate isomerase gene) and alsE (allulose 6-phosphate epimerase gene) were Als -. Transcription of the two allose operons, measured as β-galactosidase activity specified by alsI-lacZ + or alsE-lacZ + operon fusions, was induced by allose. Ribose also caused derepression of expression of the regulon under conditions in which ribose phosphate catabolism was impaired. ",

author = "Poulsen, \{Tim S.\} and Ying-Ying Chang and Bjarne Hove-Jensen",

year = "1999",

month = nov,

doi = "10.1128/jb.181.22.7126-7130.1999",

language = "English",

volume = "181",

pages = "7126--7130",

journal = "Journal of Bacteriology",

issn = "0021-9193",

publisher = "American Society for Microbiology",

number = "22",

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TY - JOUR

T1 - D-Allose catabolism of Escherichia coli

T2 - Involvement of alsI and regulation of als regulon expression by allose and ribose

AU - Poulsen, Tim S.

AU - Chang, Ying-Ying

AU - Hove-Jensen, Bjarne

PY - 1999/11

Y1 - 1999/11

N2 - Genes involved in allose utilization of Escherichia coli K-12 are organized in at least two operons, alsRBACE and alsI, located next to each other on the chromosome but divergently transcribed. Mutants defective in alsI (allose 6-phosphate isomerase gene) and alsE (allulose 6-phosphate epimerase gene) were Als -. Transcription of the two allose operons, measured as β-galactosidase activity specified by alsI-lacZ + or alsE-lacZ + operon fusions, was induced by allose. Ribose also caused derepression of expression of the regulon under conditions in which ribose phosphate catabolism was impaired.

AB - Genes involved in allose utilization of Escherichia coli K-12 are organized in at least two operons, alsRBACE and alsI, located next to each other on the chromosome but divergently transcribed. Mutants defective in alsI (allose 6-phosphate isomerase gene) and alsE (allulose 6-phosphate epimerase gene) were Als -. Transcription of the two allose operons, measured as β-galactosidase activity specified by alsI-lacZ + or alsE-lacZ + operon fusions, was induced by allose. Ribose also caused derepression of expression of the regulon under conditions in which ribose phosphate catabolism was impaired.

UR - https://www.scopus.com/pages/publications/0032748206

U2 - 10.1128/jb.181.22.7126-7130.1999

DO - 10.1128/jb.181.22.7126-7130.1999

M3 - Journal article

SN - 0021-9193

VL - 181

SP - 7126

EP - 7130

JO - Journal of Bacteriology

JF - Journal of Bacteriology

IS - 22

ER -

D-Allose catabolism of Escherichia coli: Involvement of alsI and regulation of als regulon expression by allose and ribose

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