Enzymatic synthesis of DNA strands containing α-L-LNA (α-L-configured locked nucleic acid) thymine nucleotides

Torben René Højland, Rakesh N Veedu, Birte Vester, Jesper Wengel

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Abstract

We describe the first enzymatic incorporation of an α-L-LNA nucleotide into an oligonucleotide. It was found that the 5'-triphosphate of α-L-LNA is a substrate for the DNA polymerases KOD, 9°N(m), Phusion and HIV RT. Three dispersed α-L-LNA thymine nucleotides can be incorporated into DNA strands by all four polymerases, but they were unable to perform consecutive incorporations of α-L-LNA nucleotides. In addition it was found that primer extension can be achieved using templates containing one α-L-LNA nucleotide.
Original languageUndefined/Unknown
JournalArtificial DNA, PNA & XNA
ISSN1949-095X
DOIs
Publication statusPublished - 2012
Externally publishedYes

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