Excitatory and inhibitory pathways modulate kainate excitotoxicity in hippocampal slice cultures

P Casaccia-Bonnefil; E Benedikz; R Rai; P J Bergold

Excitatory and inhibitory pathways modulate kainate excitotoxicity in hippocampal slice cultures

P Casaccia-Bonnefil
, E Benedikz
, R Rai
, P J Bergold

Department of Pharmacology, SUNY-HSCB, Brooklyn 11203.
State University of New York

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

In organotypic hippocampal slice cultures, kainate (KA) specifically induces cell loss in the CA3 region while N-methyl-D-aspartate induces cell loss in the CA1 region. The sensitivity of slice cultures to KA toxicity appears only after 2 weeks in vitro which parallels the appearance of mossy fibers. KA toxicity is potentiated by co-application with the GABA-A antagonist, picrotoxin. These data suggest that the excitotoxicity of KA in slice cultures is modulated by both excitatory and inhibitory synapses.

Original language	English
Journal	Neuroscience Letters
Volume	154
Issue number	1-2
Pages (from-to)	5-8
Number of pages	4
ISSN	0304-3940
Publication status	Published - 14 May 1993
Externally published	Yes

Keywords

Animals
Animals, Newborn
Hippocampus
Journal Article
Kainic Acid
N-Methylaspartate
Nerve Fibers
Neural Pathways
Neurons
Organ Culture Techniques
Picrotoxin
Rats
Rats, Sprague-Dawley
Research Support, Non-U.S. Gov't
Synapses

Cite this

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title = "Excitatory and inhibitory pathways modulate kainate excitotoxicity in hippocampal slice cultures",

abstract = "In organotypic hippocampal slice cultures, kainate (KA) specifically induces cell loss in the CA3 region while N-methyl-D-aspartate induces cell loss in the CA1 region. The sensitivity of slice cultures to KA toxicity appears only after 2 weeks in vitro which parallels the appearance of mossy fibers. KA toxicity is potentiated by co-application with the GABA-A antagonist, picrotoxin. These data suggest that the excitotoxicity of KA in slice cultures is modulated by both excitatory and inhibitory synapses.",

keywords = "Animals, Animals, Newborn, Hippocampus, Journal Article, Kainic Acid, N-Methylaspartate, Nerve Fibers, Neural Pathways, Neurons, Organ Culture Techniques, Picrotoxin, Rats, Rats, Sprague-Dawley, Research Support, Non-U.S. Gov't, Synapses",

author = "P Casaccia-Bonnefil and E Benedikz and R Rai and Bergold, \{P J\}",

year = "1993",

month = may,

day = "14",

language = "English",

volume = "154",

pages = "5--8",

journal = "Neuroscience Letters",

issn = "0304-3940",

publisher = "Elsevier Ireland Ltd",

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TY - JOUR

T1 - Excitatory and inhibitory pathways modulate kainate excitotoxicity in hippocampal slice cultures

AU - Casaccia-Bonnefil, P

AU - Benedikz, E

AU - Rai, R

AU - Bergold, P J

PY - 1993/5/14

Y1 - 1993/5/14

N2 - In organotypic hippocampal slice cultures, kainate (KA) specifically induces cell loss in the CA3 region while N-methyl-D-aspartate induces cell loss in the CA1 region. The sensitivity of slice cultures to KA toxicity appears only after 2 weeks in vitro which parallels the appearance of mossy fibers. KA toxicity is potentiated by co-application with the GABA-A antagonist, picrotoxin. These data suggest that the excitotoxicity of KA in slice cultures is modulated by both excitatory and inhibitory synapses.

AB - In organotypic hippocampal slice cultures, kainate (KA) specifically induces cell loss in the CA3 region while N-methyl-D-aspartate induces cell loss in the CA1 region. The sensitivity of slice cultures to KA toxicity appears only after 2 weeks in vitro which parallels the appearance of mossy fibers. KA toxicity is potentiated by co-application with the GABA-A antagonist, picrotoxin. These data suggest that the excitotoxicity of KA in slice cultures is modulated by both excitatory and inhibitory synapses.

KW - Animals

KW - Animals, Newborn

KW - Hippocampus

KW - Journal Article

KW - Kainic Acid

KW - N-Methylaspartate

KW - Nerve Fibers

KW - Neural Pathways

KW - Neurons

KW - Organ Culture Techniques

KW - Picrotoxin

KW - Rats

KW - Rats, Sprague-Dawley

KW - Research Support, Non-U.S. Gov't

KW - Synapses

M3 - Journal article

C2 - 8361647

SN - 0304-3940

VL - 154

SP - 5

EP - 8

JO - Neuroscience Letters

JF - Neuroscience Letters

IS - 1-2

ER -

Excitatory and inhibitory pathways modulate kainate excitotoxicity in hippocampal slice cultures

Abstract

Keywords

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