Heterogenic expression of stem cell markers in patient-derived glioblastoma spheroid cultures exposed to long-term hypoxia

Tine Rosenberg, Charlotte Aaberg-Jessen, Stine Asferg Petterson, Bjarne Winther Kristensen

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

AIM: To investigate the time profile of hypoxia and stem cell markers in glioblastoma spheroids of known molecular subtype.

MATERIALS & METHODS: Patient-derived glioblastoma spheroids were cultured up to 7 days in either 2% or 21% oxygen. Levels of proliferation (Ki-67), hypoxia (HIF-1α, CA9 and VEGF) and stem cell markers (CD133, nestin and musashi-1) were investigated by immunohistochemistry.

RESULTS: Hypoxia markers as well as CD133 and partially nestin increased in long-term hypoxia. The proliferation rate and spheroid size were highest in normoxia.

CONCLUSION: We found differences in hypoxia and stem cell marker profiles between the patient-derived glioblastoma cultures. This heterogeneity should be taken into consideration in development of future therapeutic strategies.

Original languageEnglish
JournalCNS oncology
Volume7
Issue number2
Pages (from-to)CNS15
ISSN2045-0907
DOIs
Publication statusPublished - Apr 2018

Keywords

  • AC133 Antigen/metabolism
  • Antigens, Neoplasm/metabolism
  • Biomarkers, Tumor/metabolism
  • Brain Neoplasms/metabolism
  • Carbonic Anhydrase IX/metabolism
  • Cell Hypoxia/physiology
  • Cell Proliferation/physiology
  • Gene Expression Regulation, Neoplastic
  • Glioblastoma/metabolism
  • Humans
  • Hypoxia-Inducible Factor 1, alpha Subunit/metabolism
  • Ki-67 Antigen/metabolism
  • Neoplastic Stem Cells/metabolism
  • Nestin/metabolism
  • Spheroids, Cellular/metabolism
  • Tumor Cells, Cultured
  • Vascular Endothelial Growth Factor A/metabolism

Fingerprint

Dive into the research topics of 'Heterogenic expression of stem cell markers in patient-derived glioblastoma spheroid cultures exposed to long-term hypoxia'. Together they form a unique fingerprint.

Cite this