Hyperexcitability and cell loss in kainate-treated hippocampal slice cultures

E Benedikz; P Casaccia-Bonnefil; A Stelzer; P J Bergold

Hyperexcitability and cell loss in kainate-treated hippocampal slice cultures

E Benedikz
, P Casaccia-Bonnefil
, A Stelzer
, P J Bergold

Department of Pharmacology, SUNY-HSCB, Brooklyn 11203.

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

Loss of hippocampal interneurons has been reported in patients with severe temporal lobe epilepsy and in animals treated with kainate. We investigated the relationship between KA induced epileptiform discharge and loss of interneurons in hippocampal slice cultures. Application of KA (1 microM) produced reversible epileptiform discharge without neurotoxicity. KA (5 microM), in contrast, produced irreversible epileptiform discharge and neurotoxicity, suggesting that the irreversible epileptiform discharge was required for the neuronal loss. Loss of CA3 pyramidal cells and parvalbumin-like immunoreactive (PV-I) interneurons preceded loss of somatostatin-like immunoreactive (SS-I) interneurons suggesting a different time course of KA neurotoxicity in these subpopulations of interneurons.

Original language	English
Journal	NeuroReport
Volume	5
Issue number	1
Pages (from-to)	90-2
Number of pages	3
ISSN	0959-4965
Publication status	Published - 25 Oct 1993
Externally published	Yes

Keywords

Animals
Dose-Response Relationship, Drug
Epilepsy
Evoked Potentials
Hippocampus
Immunohistochemistry
Interneurons
Journal Article
Kainic Acid
Nerve Fibers
Organ Culture Techniques
Parvalbumins
Pyramidal Cells
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.
Somatostatin
Time Factors

Cite this

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title = "Hyperexcitability and cell loss in kainate-treated hippocampal slice cultures",

abstract = "Loss of hippocampal interneurons has been reported in patients with severe temporal lobe epilepsy and in animals treated with kainate. We investigated the relationship between KA induced epileptiform discharge and loss of interneurons in hippocampal slice cultures. Application of KA (1 microM) produced reversible epileptiform discharge without neurotoxicity. KA (5 microM), in contrast, produced irreversible epileptiform discharge and neurotoxicity, suggesting that the irreversible epileptiform discharge was required for the neuronal loss. Loss of CA3 pyramidal cells and parvalbumin-like immunoreactive (PV-I) interneurons preceded loss of somatostatin-like immunoreactive (SS-I) interneurons suggesting a different time course of KA neurotoxicity in these subpopulations of interneurons.",

keywords = "Animals, Dose-Response Relationship, Drug, Epilepsy, Evoked Potentials, Hippocampus, Immunohistochemistry, Interneurons, Journal Article, Kainic Acid, Nerve Fibers, Organ Culture Techniques, Parvalbumins, Pyramidal Cells, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S., Somatostatin, Time Factors",

author = "E Benedikz and P Casaccia-Bonnefil and A Stelzer and Bergold, \{P J\}",

year = "1993",

month = oct,

day = "25",

language = "English",

volume = "5",

pages = "90--2",

journal = "NeuroReport",

issn = "0959-4965",

publisher = "Lippincott Williams and Wilkins",

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TY - JOUR

T1 - Hyperexcitability and cell loss in kainate-treated hippocampal slice cultures

AU - Benedikz, E

AU - Casaccia-Bonnefil, P

AU - Stelzer, A

AU - Bergold, P J

PY - 1993/10/25

Y1 - 1993/10/25

N2 - Loss of hippocampal interneurons has been reported in patients with severe temporal lobe epilepsy and in animals treated with kainate. We investigated the relationship between KA induced epileptiform discharge and loss of interneurons in hippocampal slice cultures. Application of KA (1 microM) produced reversible epileptiform discharge without neurotoxicity. KA (5 microM), in contrast, produced irreversible epileptiform discharge and neurotoxicity, suggesting that the irreversible epileptiform discharge was required for the neuronal loss. Loss of CA3 pyramidal cells and parvalbumin-like immunoreactive (PV-I) interneurons preceded loss of somatostatin-like immunoreactive (SS-I) interneurons suggesting a different time course of KA neurotoxicity in these subpopulations of interneurons.

AB - Loss of hippocampal interneurons has been reported in patients with severe temporal lobe epilepsy and in animals treated with kainate. We investigated the relationship between KA induced epileptiform discharge and loss of interneurons in hippocampal slice cultures. Application of KA (1 microM) produced reversible epileptiform discharge without neurotoxicity. KA (5 microM), in contrast, produced irreversible epileptiform discharge and neurotoxicity, suggesting that the irreversible epileptiform discharge was required for the neuronal loss. Loss of CA3 pyramidal cells and parvalbumin-like immunoreactive (PV-I) interneurons preceded loss of somatostatin-like immunoreactive (SS-I) interneurons suggesting a different time course of KA neurotoxicity in these subpopulations of interneurons.

KW - Animals

KW - Dose-Response Relationship, Drug

KW - Epilepsy

KW - Evoked Potentials

KW - Hippocampus

KW - Immunohistochemistry

KW - Interneurons

KW - Journal Article

KW - Kainic Acid

KW - Nerve Fibers

KW - Organ Culture Techniques

KW - Parvalbumins

KW - Pyramidal Cells

KW - Research Support, Non-U.S. Gov't

KW - Research Support, U.S. Gov't, P.H.S.

KW - Somatostatin

KW - Time Factors

M3 - Journal article

C2 - 7904192

SN - 0959-4965

VL - 5

SP - 90

EP - 92

JO - NeuroReport

JF - NeuroReport

IS - 1

ER -

Hyperexcitability and cell loss in kainate-treated hippocampal slice cultures

Abstract

Keywords

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