Bacillus caldolyticus prs gene encoding phosphoribosyl-diphosphate synthase

Britta N. Krath; Bjarne Hove-Jensen

doi:10.1016/0378-1119(96)00222-3

Bacillus caldolyticus prs gene encoding phosphoribosyl-diphosphate synthase

Britta N. Krath
, Bjarne Hove-Jensen

University of Copenhagen

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

The prs gene, encoding phosphoribosyl-diphosphate (PRPP) synthase, as well as the flanking DNA Sequences were cloned and sequenced from the Gram-positive thermophile, Bacillus caldolyticus. Comparison with the homologous sequences from the mesophile, Bacillus subtilis, revealed a gene (gcaD) encoding N-acetylglucosamine-1-phosphate uridyltransferase upstream of prs and a gene homologous to etc downstream of prs. cDNA synthesis with a B. caldolyticus gcaD-prs-ctc-specified mRNA as template, followed by amplification utilising the polymerase chain reaction indicated that the three genes are co-transcribed. Comparison of amino acid sequences revealed a high similarity among PRPP synthases across a wide phylogenetic range. An E. coli strain harbouring the B. caldolyticus prs gene in a multicopy plasmid produced PRPP synthase activity 33-fold over the activity of a haploid B. caldolyticus strain. B. caldolyticus PRPP synthase was resistant to heat treatment at 70°C to a much higher extent than PRPP synthase from B. subtilis.

Original language	English
Journal	Gene
Volume	176
Issue number	1-2
Pages (from-to)	73-79
Number of pages	7
ISSN	0378-1119
DOIs	https://doi.org/10.1016/0378-1119(96)00222-3
Publication status	Published - 17 Oct 1996

Keywords

CDNA
N-acetylglucosamine-1-phosphate uridyltransferase
Operon expression
Phosphoribosyl diphosphate
Phosphoribosylpyro-phosphate
Thermophile
ctc
gcaD
tms

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10.1016/0378-1119(96)00222-3

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@article{de60c6a89bb448fab9a8003604785c2f,

title = "Bacillus caldolyticus prs gene encoding phosphoribosyl-diphosphate synthase",

abstract = "The prs gene, encoding phosphoribosyl-diphosphate (PRPP) synthase, as well as the flanking DNA Sequences were cloned and sequenced from the Gram-positive thermophile, Bacillus caldolyticus. Comparison with the homologous sequences from the mesophile, Bacillus subtilis, revealed a gene (gcaD) encoding N-acetylglucosamine-1-phosphate uridyltransferase upstream of prs and a gene homologous to etc downstream of prs. cDNA synthesis with a B. caldolyticus gcaD-prs-ctc-specified mRNA as template, followed by amplification utilising the polymerase chain reaction indicated that the three genes are co-transcribed. Comparison of amino acid sequences revealed a high similarity among PRPP synthases across a wide phylogenetic range. An E. coli strain harbouring the B. caldolyticus prs gene in a multicopy plasmid produced PRPP synthase activity 33-fold over the activity of a haploid B. caldolyticus strain. B. caldolyticus PRPP synthase was resistant to heat treatment at 70°C to a much higher extent than PRPP synthase from B. subtilis.",

keywords = "CDNA, N-acetylglucosamine-1-phosphate uridyltransferase, Operon expression, Phosphoribosyl diphosphate, Phosphoribosylpyro-phosphate, Thermophile, ctc, gcaD, tms",

author = "Krath, \{Britta N.\} and Bjarne Hove-Jensen",

year = "1996",

month = oct,

day = "17",

doi = "10.1016/0378-1119(96)00222-3",

language = "English",

volume = "176",

pages = "73--79",

journal = "Gene",

issn = "0378-1119",

publisher = "Elsevier B.V.",

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TY - JOUR

T1 - Bacillus caldolyticus prs gene encoding phosphoribosyl-diphosphate synthase

AU - Krath, Britta N.

AU - Hove-Jensen, Bjarne

PY - 1996/10/17

Y1 - 1996/10/17

N2 - The prs gene, encoding phosphoribosyl-diphosphate (PRPP) synthase, as well as the flanking DNA Sequences were cloned and sequenced from the Gram-positive thermophile, Bacillus caldolyticus. Comparison with the homologous sequences from the mesophile, Bacillus subtilis, revealed a gene (gcaD) encoding N-acetylglucosamine-1-phosphate uridyltransferase upstream of prs and a gene homologous to etc downstream of prs. cDNA synthesis with a B. caldolyticus gcaD-prs-ctc-specified mRNA as template, followed by amplification utilising the polymerase chain reaction indicated that the three genes are co-transcribed. Comparison of amino acid sequences revealed a high similarity among PRPP synthases across a wide phylogenetic range. An E. coli strain harbouring the B. caldolyticus prs gene in a multicopy plasmid produced PRPP synthase activity 33-fold over the activity of a haploid B. caldolyticus strain. B. caldolyticus PRPP synthase was resistant to heat treatment at 70°C to a much higher extent than PRPP synthase from B. subtilis.

AB - The prs gene, encoding phosphoribosyl-diphosphate (PRPP) synthase, as well as the flanking DNA Sequences were cloned and sequenced from the Gram-positive thermophile, Bacillus caldolyticus. Comparison with the homologous sequences from the mesophile, Bacillus subtilis, revealed a gene (gcaD) encoding N-acetylglucosamine-1-phosphate uridyltransferase upstream of prs and a gene homologous to etc downstream of prs. cDNA synthesis with a B. caldolyticus gcaD-prs-ctc-specified mRNA as template, followed by amplification utilising the polymerase chain reaction indicated that the three genes are co-transcribed. Comparison of amino acid sequences revealed a high similarity among PRPP synthases across a wide phylogenetic range. An E. coli strain harbouring the B. caldolyticus prs gene in a multicopy plasmid produced PRPP synthase activity 33-fold over the activity of a haploid B. caldolyticus strain. B. caldolyticus PRPP synthase was resistant to heat treatment at 70°C to a much higher extent than PRPP synthase from B. subtilis.

KW - CDNA

KW - N-acetylglucosamine-1-phosphate uridyltransferase

KW - Operon expression

KW - Phosphoribosyl diphosphate

KW - Phosphoribosylpyro-phosphate

KW - Thermophile

KW - ctc

KW - gcaD

KW - tms

UR - https://www.scopus.com/pages/publications/0030591757

U2 - 10.1016/0378-1119(96)00222-3

DO - 10.1016/0378-1119(96)00222-3

M3 - Journal article

SN - 0378-1119

VL - 176

SP - 73

EP - 79

JO - Gene

JF - Gene

IS - 1-2

ER -

Bacillus caldolyticus prs gene encoding phosphoribosyl-diphosphate synthase

Abstract

Keywords

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