Metabolic engineering of yeast for fermentative production of flavonoids

Angelica Rodriguez; Tomas Strucko; Steen Gustav Stahlhut; Mette Kristensen; Daniel Killerup Svenssen; Jochen Forster; Jens Nielsen; Irina Borodina

doi:10.1016/j.biortech.2017.06.043

Metabolic engineering of yeast for fermentative production of flavonoids

Angelica Rodriguez
, Tomas Strucko
, Steen Gustav Stahlhut
, Mette Kristensen
, Daniel Killerup Svenssen
, Jochen Forster
, Jens Nielsen
, Irina Borodina

Technical University of Denmark

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

Yeast Saccharomyces cerevisiae was engineered for de novo production of six different flavonoids (naringenin, liquiritigenin, kaempferol, resokaempferol, quercetin, and fisetin) directly from glucose, without supplementation of expensive intermediates. This required reconstruction of long biosynthetic pathways, comprising up to eight heterologous genes from plants. The obtained titers of kaempferol 26.57 ± 2.66 mg L−1and quercetin 20.38 ± 2.57 mg L−1exceed the previously reported titers in yeast. This is also the first report of de novo biosynthesis of resokaempferol and fisetin in yeast. The work demonstrates the potential of flavonoid-producing yeast cell factories.

Original language	English
Journal	Bioresource Technology
Volume	245
Issue number	Pt B
Pages (from-to)	1645-1654
Number of pages	10
ISSN	0960-8524
DOIs	https://doi.org/10.1016/j.biortech.2017.06.043
Publication status	Published - 1 Dec 2017
Externally published	Yes

Keywords

Biosynthetic Pathways
Fermentation
Flavonoids
Metabolic Engineering
Saccharomyces cerevisiae

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10.1016/j.biortech.2017.06.043

Cite this

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title = "Metabolic engineering of yeast for fermentative production of flavonoids",

abstract = "Yeast Saccharomyces cerevisiae was engineered for de novo production of six different flavonoids (naringenin, liquiritigenin, kaempferol, resokaempferol, quercetin, and fisetin) directly from glucose, without supplementation of expensive intermediates. This required reconstruction of long biosynthetic pathways, comprising up to eight heterologous genes from plants. The obtained titers of kaempferol 26.57 ± 2.66 mg L−1and quercetin 20.38 ± 2.57 mg L−1exceed the previously reported titers in yeast. This is also the first report of de novo biosynthesis of resokaempferol and fisetin in yeast. The work demonstrates the potential of flavonoid-producing yeast cell factories.",

keywords = "Biosynthetic Pathways, Fermentation, Flavonoids, Metabolic Engineering, Saccharomyces cerevisiae",

author = "Angelica Rodriguez and Tomas Strucko and Stahlhut, \{Steen Gustav\} and Mette Kristensen and Svenssen, \{Daniel Killerup\} and Jochen Forster and Jens Nielsen and Irina Borodina",

year = "2017",

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doi = "10.1016/j.biortech.2017.06.043",

language = "English",

volume = "245",

pages = "1645--1654",

journal = "Bioresource Technology",

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TY - JOUR

T1 - Metabolic engineering of yeast for fermentative production of flavonoids

AU - Rodriguez, Angelica

AU - Strucko, Tomas

AU - Stahlhut, Steen Gustav

AU - Kristensen, Mette

AU - Svenssen, Daniel Killerup

AU - Forster, Jochen

AU - Nielsen, Jens

AU - Borodina, Irina

PY - 2017/12/1

Y1 - 2017/12/1

N2 - Yeast Saccharomyces cerevisiae was engineered for de novo production of six different flavonoids (naringenin, liquiritigenin, kaempferol, resokaempferol, quercetin, and fisetin) directly from glucose, without supplementation of expensive intermediates. This required reconstruction of long biosynthetic pathways, comprising up to eight heterologous genes from plants. The obtained titers of kaempferol 26.57 ± 2.66 mg L−1and quercetin 20.38 ± 2.57 mg L−1exceed the previously reported titers in yeast. This is also the first report of de novo biosynthesis of resokaempferol and fisetin in yeast. The work demonstrates the potential of flavonoid-producing yeast cell factories.

AB - Yeast Saccharomyces cerevisiae was engineered for de novo production of six different flavonoids (naringenin, liquiritigenin, kaempferol, resokaempferol, quercetin, and fisetin) directly from glucose, without supplementation of expensive intermediates. This required reconstruction of long biosynthetic pathways, comprising up to eight heterologous genes from plants. The obtained titers of kaempferol 26.57 ± 2.66 mg L−1and quercetin 20.38 ± 2.57 mg L−1exceed the previously reported titers in yeast. This is also the first report of de novo biosynthesis of resokaempferol and fisetin in yeast. The work demonstrates the potential of flavonoid-producing yeast cell factories.

KW - Biosynthetic Pathways

KW - Fermentation

KW - Flavonoids

KW - Metabolic Engineering

KW - Saccharomyces cerevisiae

UR - http://www.mendeley.com/research/metabolic-engineering-yeast-fermentative-production-flavonoids

U2 - 10.1016/j.biortech.2017.06.043

DO - 10.1016/j.biortech.2017.06.043

M3 - Journal article

C2 - 28634125

SN - 0960-8524

VL - 245

SP - 1645

EP - 1654

JO - Bioresource Technology

JF - Bioresource Technology

IS - Pt B

ER -

Metabolic engineering of yeast for fermentative production of flavonoids

Abstract

Keywords

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