Methods for Isolation, Purification, and Propagation of Bacteriophages of Campylobacter jejuni

Yilmaz Emre Gencay, Tina Birk, Martine Camilla Holst Sørensen, Lone Brøndsted

Research output: Chapter in Book/Report/Conference proceedingContribution to book/anthologyResearchpeer-review

Abstract

Here, we describe the methods for isolation, purification, and propagation of Campylobacter jejuni bacteriophages from samples expected to contain high number of phages such as chicken feces. The overall steps are (1) liberation of phages from the sample material; (2) observation of plaque-forming units on C. jejuni lawns using a spot assay; (3) isolation of single plaques; (4) consecutive purification procedures; and (5) propagation of purified phages from a plate lysate to prepare master stocks.
Original languageDanish
Title of host publicationCampylobacter jejuni - methods and protocols
EditorsJames Butcher, Alain Stintzi
Number of pages10
PublisherSpringer Science+Business Media
Publication date2017
Pages19-28
ISBN (Print)978-1-4939-6534-2
Publication statusPublished - 2017
Externally publishedYes

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