Phage-display libraries of murine and human antibody Fab fragments: Protocol

Jan Engberg; Peter Sejer Andersen; Leif Kofoed Nielsen; Morten Hanefeld Dziegiel; Lene Karin Johansen; Bjarne Albrechtsen

doi:10.1007/BF02761708

Phage-display libraries of murine and human antibody Fab fragments: Protocol

Jan Engberg
, Peter Sejer Andersen
, Leif Kofoed Nielsen
, Morten Hanefeld Dziegiel
, Lene Karin Johansen
, Bjarne Albrechtsen

Royal Danish School of Pharmacy, Department of Biology
University of Copenhagen
Kennedy Center

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

We provide efficient and detailed procedures for construction, expression, and screening of comprehensive libraries of murine or human antibody Fab fragments displayed on the surface of filamentous phage. In addition, protocols for producing and using ultra-electrocompetent cells, for producing Fab phages from libraries, and for selecting antigen binders by panning are presented. The latter protocol includes a procedure for trypsin elution of bound phage.

Original language	English
Journal	Molecular Biotechnology
Volume	6
Issue number	3
Pages (from-to)	287-310
Number of pages	24
ISSN	1073-6085
DOIs	https://doi.org/10.1007/BF02761708
Publication status	Published - Dec 1996
Externally published	Yes

Keywords

clinical assessment methods, lab technology and radiography
disease, health science and nursing

Access to Document

10.1007/BF02761708

Cite this

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title = "Phage-display libraries of murine and human antibody Fab fragments: Protocol",

abstract = "We provide efficient and detailed procedures for construction, expression, and screening of comprehensive libraries of murine or human antibody Fab fragments displayed on the surface of filamentous phage. In addition, protocols for producing and using ultra-electrocompetent cells, for producing Fab phages from libraries, and for selecting antigen binders by panning are presented. The latter protocol includes a procedure for trypsin elution of bound phage.",

keywords = "clinical assessment methods, lab technology and radiography, disease, health science and nursing",

author = "Jan Engberg and Andersen, \{Peter Sejer\} and Nielsen, \{Leif Kofoed\} and Dziegiel, \{Morten Hanefeld\} and Johansen, \{Lene Karin\} and Bjarne Albrechtsen",

year = "1996",

month = dec,

doi = "10.1007/BF02761708",

language = "English",

volume = "6",

pages = "287--310",

journal = "Molecular Biotechnology",

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T1 - Phage-display libraries of murine and human antibody Fab fragments

T2 - Protocol

AU - Engberg, Jan

AU - Andersen, Peter Sejer

AU - Nielsen, Leif Kofoed

AU - Dziegiel, Morten Hanefeld

AU - Johansen, Lene Karin

AU - Albrechtsen, Bjarne

PY - 1996/12

Y1 - 1996/12

N2 - We provide efficient and detailed procedures for construction, expression, and screening of comprehensive libraries of murine or human antibody Fab fragments displayed on the surface of filamentous phage. In addition, protocols for producing and using ultra-electrocompetent cells, for producing Fab phages from libraries, and for selecting antigen binders by panning are presented. The latter protocol includes a procedure for trypsin elution of bound phage.

AB - We provide efficient and detailed procedures for construction, expression, and screening of comprehensive libraries of murine or human antibody Fab fragments displayed on the surface of filamentous phage. In addition, protocols for producing and using ultra-electrocompetent cells, for producing Fab phages from libraries, and for selecting antigen binders by panning are presented. The latter protocol includes a procedure for trypsin elution of bound phage.

KW - clinical assessment methods, lab technology and radiography

KW - disease, health science and nursing

U2 - 10.1007/BF02761708

DO - 10.1007/BF02761708

M3 - Journal article

SN - 1073-6085

VL - 6

SP - 287

EP - 310

JO - Molecular Biotechnology

JF - Molecular Biotechnology

IS - 3

ER -

Phage-display libraries of murine and human antibody Fab fragments: Protocol

Abstract

Keywords

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